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The melting temperature (\(T_m\)) of DNA is

Question and options

The melting temperature (TmT_m) of DNA is
  1. directly proportional to the length of the DNA chain
  2. directly proportional to the GC content
  3. directly proportional to the AT content
  4. not related to the base composition

Answer and explanation

Correct answer: B - directly proportional to the GC content
GC pairs have three hydrogen bonds (and stronger stacking) versus two for AT, so TmT_m increases with GC content.

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